Fractionation of Soybean Proteins by Column Chromatography on DEAE-Cellulose

Separation of folate binding protein from human serum by DEAE-cellulose column chromatography.

On diethylaminoethyl-cellulose column chromatography, the folate binding protein in the serum of 21 patients eluted in the early effluents as a single sharply defined peak. The chromatographic behavior of the folate binder remained unchanged whether or not the serum was, before chromatography, complexed with tritium-labeled pteroylglutamic acid ([3H]PGA), dialyzed, or charcoal-adsorbed. Heating...

Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of hepatobiliary diseases.

Using chromatography on diethylaminoethyl (DEAE) cellulose, we measured biliary alkaline phosphatase (BALP; EC 3.1.3.1) activities in sera from 182 patients, most with hepatobiliary disorders but some with non-hepatobiliary diseases. Relative BALP activities were extremely low in otherwise healthy carriers of hepatitis B virus (mean: 5.4 U/L) and in patients with non-hepatobiliary diseases (mea...

Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.

Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutin...

Analytical separations of metals by column chromatography on microcrystalline cellulose

Separation of vitamin B 12-binding proteins of serum, gastric juice and saliva by rapid DEAE cellulose chromatography.

By FRANCOIS P. RETIEF, CHESTER W. GOTFLIEB, SHAUL KOCHWA, PETER W. PRATF AND VICTOR HERBERT N ORMAL SERUM contains at least two binders of vitamin B12, one migrating electrophoretically as an alpha-globulin2’3 and the other as a beta-globulin.46 There is evidence that these substances have different metabolic functions. Whereas native B12 is bound to alpha-globulin,2 the betaglobulin binder pro...